Analysis Note
ControlIrradiated normal Human fibroblasts (no reactivity against non-irradiated cell extracts)
Application
Anti-ATM Antibody, phosphoSer1981, clone 10H11.E12 is a Mouse Monoclonal Antibody for detection of ATM also known as Ataxia Telangiectasia Mutated & has been validated in IP, WB, ICC.
Research CategoryEpigenetics & Nuclear Function
Western blot: Detects a 370 kDa protein in crude extracts from irradiated human foreskin or mouse 3T3L1 cells.
Immunocytochemistry: Foci are detected in irradiated human and mouse fibroblasts.
Immunoprecipitation: The antibody immunoprecipitates ATM from irradiated human and mouse cells.
Optimal working dilutions must be determined by end user.
Research Sub CategoryCell Cycle, DNA Replication & Repair
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). Ataxia telangiectasia (AT) is a debilitating neurodegenerative disease that occurs early in childhood, resulting in ataxic movements and speech defects caused by cerebellar degeneration. The underlying cause of the disease is a biochemical dysfunction in the cellular response to specific types of DNA damage, correlated with mutations in the protein kinase ATM (Ataxia telangiectasia mutated). ATM not only is key in the signaling cascade that responds to DNA double-stranded breaks, but it also controls cell-cycle checkpoints and is therefore important in cancer prevention.
Immunogen
Synthetic peptide corresponding to amino acids 1974-1988 of human ATM (SLAFEEG[pS]QSTTISS).
Epitope: phosphoSer1981
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin presented as a liquid in 0.02M phosphate buffer containing 0.25M NaCl and 0.1% sodium azide.
Specificity
Reacts with ATM Kinase phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
This antibody and certain aspects of its use are disclosed and claimed in pending U.S. Patent Applications published as U.S. Patent Publication Nos. 2003/0077661 and 2003/0157572.
This product has met the following criteria to qualify for the following awards: